Non-ribosomal peptide synthetases catalyze the biosynthesis of structurally and functionally diverse non-ribosomal peptide natural products, which have broad applications in pharmaceutical, agricultural, and industrial sectors. Engineered non-ribosomal peptide synthetases can be used to produce novel non-ribosomal peptides through combinatorial biosynthesis. This conforms to the concept of green chemistry, thus attracts increasing attention across the world. Herein, three different engineering strategies were summarized, and recent advances in this field were reviewed.
非核糖体肽合成酶合成的非核糖体肽类天然产物具有丰富的结构和多样的功能，在医药、农业、工业等领域具有广泛的应用潜力。利用合成生物技术工程改造非核糖体肽合成酶，在微生物细胞工厂中组合生物合成新型非核糖体肽分子顺应绿色化学的发展理念，是国内外学者关注的热点。文中归纳了3种不同的非核糖体肽合成酶工程改造策略，并对近年来相关领域的研究进展进行综述。.
Keywords: combinatorial biosynthesis; domain swap; engineering; non-ribosomal peptide synthetase; synthetic biology techniques.