Characterization of Vascular plant One-Zinc finger (VOZ) in soybean (Glycine max and Glycine soja) and their expression analyses under drought condition

Shoaib Ur Rehman; Ghulam Qanmber; Muhammad Hammad Nadeem Tahir; Ahsan Irshad; Sajid Fiaz; Furqan Ahmad; Zulfiqar Ali; Muhammad Sajjad; Muhammad Shees; Muhammad Usman; Zhide Geng

doi:10.1371/journal.pone.0253836

Characterization of Vascular plant One-Zinc finger (VOZ) in soybean (Glycine max and Glycine soja) and their expression analyses under drought condition

PLoS One. 2021 Jul 2;16(7):e0253836. doi: 10.1371/journal.pone.0253836. eCollection 2021.

Authors

Affiliations

¹ Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef University of Agriculture Multan, Multan, Pakistan.
² State Key Laboratory of Cotton Biology, Cotton Research Institute of Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
³ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Engineering Laboratory of Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
⁴ Department of Plant Breeding and Genetics, The University of Haripur, Haripur, Pakistan.
⁵ Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
⁶ Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad, Pakistan.
⁷ Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, China.

Abstract

Vascular plant one-zinc-finger (VOZ) transcription factors regulate plant growth and development under drought conditions. Six VOZ transcription factors encoding genes exist in soybean genome (both in Glycine max and Glycine soja). Herein, GmVOZs and GsVOZs were identified through in silico analysis and characterized with different bioinformatics tools and expression analysis. Phylogenetic analysis classified VOZ genes in four groups. Sequence logos analysis among G. max and G. soja amino acid residues revealed higher conservation. Presence of stress related cis-elements in the upstream regions of GmVOZs and GsVOZs highlights their role in tolerance against abiotic stresses. The collinearity analysis identified 14 paralogous/orthologous gene pairs within and between G. max and G. soja. The Ka/Ks values showed that soybean VOZ genes underwent selection pressure with limited functional deviation arising from whole genome and segmental duplication. The GmVOZs and GsVOZs were found to express in roots and leaves at seedling stage. The qRT-PCR revealed that GmVOZs and GsVOZs transcripts can be regulated by abiotic stresses such as polyethylene glycol (PEG). The findings of this study will provide a reference to decipher physiological and molecular functions of VOZ genes in soybean.

Publication types

Research Support, Non-U.S. Gov't
Retracted Publication

MeSH terms

Acclimatization / genetics*
Amino Acid Sequence / genetics
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Droughts
Gene Expression Regulation, Plant*
Glycine max / physiology*
Plant Leaves
Plant Proteins / genetics*
Plant Proteins / metabolism
Plant Roots
Seedlings
Sequence Alignment
Stress, Physiological / genetics
Transcription Factors / genetics*
Transcription Factors / metabolism
Zinc Fingers

Substances

DNA-Binding Proteins
Plant Proteins
Transcription Factors

Grants and funding

This research was funded by Pakistan Agricultural Research Board, project number PARB-830 and South and Southeast Asia Agricultural Science and Technology Radiation Center. Grant Number 2018IA086.