Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

Muhammad Salman Haider; Savitha De Britto; Geetha Nagaraj; Bhavya Gurulingaiah; Ravikant Shekhar; Shin-Ichi Ito; Sudisha Jogaiah

doi:10.3390/ijms22126585

Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

Int J Mol Sci. 2021 Jun 19;22(12):6585. doi: 10.3390/ijms22126585.

Authors

Muhammad Salman Haider¹, Savitha De Britto^{2

3}, Geetha Nagaraj⁴, Bhavya Gurulingaiah⁴, Ravikant Shekhar⁴, Shin-Ichi Ito^{5

6}, Sudisha Jogaiah²

Affiliations

¹ Key Laboratory of Genetics and Fruit Development, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
² Laboratory of Plant Healthcare and Diagnostics, P.G. Department of Biotechnology and Microbiology, Karnatak University, Dharwad 580003, India.
³ Division of Biological Sciences, School of Science and Technology, University of Goroka, Goroka 441, Papua New Guinea.
⁴ Nanobiotechnology Laboratory, Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 570006, India.
⁵ Laboratory of Molecular Plant Pathology, Department of Biological and Environmental Sciences, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan.
⁶ Research Center for Thermotolerant Microbial Resources (RCTMR), Yamaguchi University, Yamaguchi 753-8515, Japan.

Abstract

Members of the lectin receptor-like kinase (LecRLKs) family play a vital role in innate plant immunity. Few members of the LecRLKs family have been characterized in rice and Arabidopsis, respectively. However, little literature is available about LecRLKs and their role against fungal infection in cucumber. In this study, 60 putative cucumber LecRLK (CsLecRLK) proteins were identified using genome-wide analysis and further characterized into L-type LecRLKs (24) and G-type LecRLKs (36) based on domain composition and phylogenetic analysis. These proteins were allocated to seven cucumber chromosomes and found to be involved in the expansion of the CsLecRLK gene family. Subcellular localization of CsaLecRLK9 and CsaLecRLK12 showed green fluorescence signals in the plasma membrane of leaves. The transcriptional profiling of CsLecRLK genes showed that L-type LecRLKs exhibited functional redundancy as compared to G-type LecRLKs. The qRT-PCR results indicated that both L- and G-type LecRLKs showed significant response against plant growth-promoting fungi (PGPF-Trichoderma harzianum Rifai), powdery mildew pathogen (PPM-Golovinomyces orontii (Castagne) V.P. Heluta), and combined (PGPF+PPM) treatments. The findings of this study contribute to a better understanding of the role of cucumber CsLecRLK genes in response to PGPF, PPM, and PGPF+PPM treatments and lay the basis for the characterization of this important functional gene family.

Keywords: biotic stress; cucumber; lectin receptor-like kinases; plant growth-promoting fungi.

Publication types

Validation Study

MeSH terms

Chromosomes, Plant
Cucumis sativus / enzymology*
Cucumis sativus / genetics
Cucumis sativus / immunology
Erysiphe / immunology*
Gene Expression Profiling
Genes, Plant
Multigene Family
Phylogeny
Plant Immunity*
Plant Proteins / genetics
Plant Proteins / metabolism
Protein Kinases / genetics*
Protein Kinases / metabolism
Stress, Physiological*

Substances

Plant Proteins
Protein Kinases