In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer

Cell Rep Med. 2021 Jun 15;2(6):100318. doi: 10.1016/j.xcrm.2021.100318.

Abstract

Endometrial cancer (EC) is one of the most common gynecological cancers worldwide. Sentinel lymph node (SLN) status could be a major prognostic factor in evaluation of EC, but several prospective studies need to be performed. Here we report an in-depth proteomics analysis showing significant variations in the SLN protein landscape in EC. We show that SLNs are correlated to each tumor grade, which strengthens evidence of SLN involvement in EC. A few proteins are overexpressed specifically at each EC tumor grade and in the corresponding SLN. These proteins, which are significantly variable in both locations, should be considered potential markers of overall survival. Five major proteins for EC and SLN (PRSS3, PTX3, ASS1, ALDH2, and ANXA1) were identified in large-scale proteomics and validated by immunohistochemistry. This study improves stratification and diagnosis of individuals with EC as a result of proteomics profiling of SLNs.

Trial registration: ClinicalTrials.gov NCT02598219.

Keywords: alternative proteins; diagnosis; endometrial cancer; ghost proteome; protein mutations; proteomics; sentinel lymph nodes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehyde Dehydrogenase, Mitochondrial / genetics*
  • Aldehyde Dehydrogenase, Mitochondrial / metabolism
  • Amino Acid Sequence
  • Annexin A1 / genetics*
  • Annexin A1 / metabolism
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism
  • C-Reactive Protein / genetics*
  • C-Reactive Protein / metabolism
  • Endometrial Neoplasms / diagnosis
  • Endometrial Neoplasms / genetics*
  • Endometrial Neoplasms / mortality
  • Endometrial Neoplasms / pathology
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Lymph Node Excision / methods
  • Lymphatic Metastasis
  • Neoplasm Grading
  • Prognosis
  • Prospective Studies
  • Proteomics / methods
  • Sentinel Lymph Node / metabolism*
  • Sentinel Lymph Node / pathology
  • Sentinel Lymph Node / surgery
  • Sentinel Lymph Node Biopsy / methods
  • Serum Amyloid P-Component / genetics*
  • Serum Amyloid P-Component / metabolism
  • Signal Transduction
  • Survival Analysis
  • Trypsin / genetics*
  • Trypsin / metabolism

Substances

  • ANXA1 protein, human
  • Annexin A1
  • Biomarkers, Tumor
  • Serum Amyloid P-Component
  • PTX3 protein
  • C-Reactive Protein
  • ALDH2 protein, human
  • Aldehyde Dehydrogenase, Mitochondrial
  • PRSS3 protein, human
  • Trypsin

Associated data

  • ClinicalTrials.gov/NCT02598219