Eight genes are necessary and sufficient for biogenesis of quinohemoprotein amine dehydrogenase

Biosci Biotechnol Biochem. 2021 Aug 25;85(9):2026-2029. doi: 10.1093/bbb/zbab117.

Abstract

Quinohemoprotein amine dehydrogenase (QHNDH) containing a peptidyl quinone cofactor, cysteine tryptophylquinone, is produced in the periplasm of Gram-negative bacteria through an intricate process of post-translational modification that requires at least 8 genes including those encoding 3 nonidentical subunits and 3 modifying enzymes. Our heterologous expression study has revealed that the 8 genes are necessary and sufficient for the QHNDH biogenesis.

Keywords: amine assimilation; biogenesis of cofactor; heterologous expression; post-translational modification; quinohemoprotein amine dehydrogenase.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Oxidoreductases Acting on CH-NH Group Donors / biosynthesis*
  • Oxidoreductases Acting on CH-NH Group Donors / chemistry
  • Oxidoreductases Acting on CH-NH Group Donors / genetics
  • Protein Conformation
  • Protein Processing, Post-Translational

Substances

  • Oxidoreductases Acting on CH-NH Group Donors
  • quinohemoprotein amine dehydrogenase