A robust CRISPR-Cas12a biosensor coated with metal-organic framework

J Mater Chem B. 2021 Jul 14;9(27):5451-5455. doi: 10.1039/d1tb01126j.

Abstract

Metal-organic frameworks (MOFs) are proposed to protect a CRISPR-associated enzyme/RNA complex from harsh environments, while the complex can be quickly released from MOFs with high efficiency. Therefore, the application of CRISPR-powered biosensing can be more feasible.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Biosensing Techniques*
  • CRISPR-Associated Proteins / metabolism*
  • CRISPR-Cas Systems / drug effects*
  • CRISPR-Cas Systems / genetics
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / pharmacology*
  • Endodeoxyribonucleases / metabolism*
  • Hydrogen-Ion Concentration
  • Metal-Organic Frameworks / chemistry
  • Metal-Organic Frameworks / pharmacology*
  • Protective Agents / chemistry
  • Protective Agents / pharmacology*
  • RNA / genetics
  • RNA / metabolism

Substances

  • Bacterial Proteins
  • CRISPR-Associated Proteins
  • Coated Materials, Biocompatible
  • Metal-Organic Frameworks
  • Protective Agents
  • RNA
  • Cas12a protein
  • Endodeoxyribonucleases