Polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production

Kexue Zhu; Haofei Fan; Shunjiang Zeng; Shaoping Nie; Yanjun Zhang; Lehe Tan; Chuan Li; Fei Xu; Qibing Liu; Gang Wu

doi:10.1016/j.foodchem.2021.130434

Polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production

Food Chem. 2021 Dec 1:364:130434. doi: 10.1016/j.foodchem.2021.130434. Epub 2021 Jun 23.

Authors

Kexue Zhu¹, Haofei Fan², Shunjiang Zeng³, Shaoping Nie⁴, Yanjun Zhang⁵, Lehe Tan⁶, Chuan Li⁷, Fei Xu⁵, Qibing Liu⁸, Gang Wu⁵

Affiliations

¹ Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China; Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning, Hainan 571533, China.
² Department of Pharmacology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, Hainan 571199, China.
³ Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China; College of Food Science and Technology, Hainan University, Haikou, Hainan 570228, China.
⁴ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
⁵ Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China.
⁶ Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China. Electronic address: tlh3687@163.com.
⁷ College of Food Science and Technology, Hainan University, Haikou, Hainan 570228, China.
⁸ Department of Pharmacology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, Hainan 571199, China. Electronic address: Qibing.liu@hainmc.edu.cn.

PMID: 34182368
DOI: 10.1016/j.foodchem.2021.130434

Abstract

This study aimed to investigate the effects of polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice. The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods. The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID). The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7. The concentrations of acetic acid, propionic acid, n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks. These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.

Keywords: Artocarpus heterophyllus Lam. pulp; Gut microbiota; Polysaccharide; Short-chain fatty acids.

MeSH terms

Animals
Artocarpus*
Fatty Acids, Volatile
Feces
Gastrointestinal Microbiome*
Mice
Polysaccharides
RNA, Ribosomal, 16S

Substances

Fatty Acids, Volatile
Polysaccharides
RNA, Ribosomal, 16S