Transient Gene Expression for the Characteristic Signal Sequences and the Estimation of the Localization of Target Protein in Plant Cell

Mikhail Berestovoy; Alexander Tyurin; Ksenia Kabardaeva; Yuriy Sidorchuk; Artem Fomenkov; Alexander Nosov; Irina Goldenkova-Pavlova

doi:10.21769/BioProtoc.2738

Transient Gene Expression for the Characteristic Signal Sequences and the Estimation of the Localization of Target Protein in Plant Cell

Bio Protoc. 2018 Feb 20;8(4):e2738. doi: 10.21769/BioProtoc.2738.

Authors

Mikhail Berestovoy^{1

2}, Alexander Tyurin^{1

2}, Ksenia Kabardaeva^{1

2}, Yuriy Sidorchuk³, Artem Fomenkov¹, Alexander Nosov¹, Irina Goldenkova-Pavlova¹

Affiliations

¹ Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia.
² Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow, Russia.
³ Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.

Abstract

We have proposed and tested a method for characterization of the signal sequences and determinations of target protein localization in a plant cell. This method, called the AgI-PrI, implies extraction of protoplasts from plant tissues after agroinfiltration. The suggested approach combines the advantages of two widely used methods for transient gene expression in plants-agroinfiltration and transfection of isolated protoplasts. The AgI-PrI technic can be applied to other plant species.

Keywords: Agroinfiltration; Protoplast isolation; Signal sequences; Subcellular localization; Tobacco; Transient expression.