Inhibitory effects of honey from arid land on some enzymes and protein damage

Abstract

Although arid land honey is outstanding for its conventional uses in food and medicine, there is an absence of data regarding its health benefits from the perspective of enzyme inhibitory effects that are affirmed by the current study. For the first time, this investigation demonstrates that different types of honey exert inhibitory effects on the activities of angiotensin, tyrosinase, xanthine oxidase, -α -amylase, acetylcholinesterase, and lipase, in addition to the inhibition of bovine serum albumin damage. The present study also provides a comparison with perceived healthy honey from non-arid areas. The results indicated huge contrasts among honey samples through all assessed parameters. Results also demonstrated that at least one type of honey from arid land contained a higher inhibition effect when compared with honey from other regions. Therefore, a possible application of arid land honey and its active compounds can be the utilization as a therapeutic agent against several diseases.

Keywords: 4-MU oleate (PubChem CID 6436487); 4-methylumbelliferone (PubChem CID 5280567); APH (PubChem CID 76344); Anti-tyrosinase; Anti-xanthine oxidase; Anti-α-amylase; Antihypertensive; BSA damage; DTNB (PubChem CID 6254); Honey; Tris-HCl buffer (PubChem CID 93573); acetylcholine iodide (PubChem CID 75271); c-holine acetyltransferase (PubChem SID 135311871); dinitrosalciylic acid (PubChem CID 11873); ethanol (PubChem CID 702); ethyl acetate (PubChem CID 8857); hippuryl-l-hestidyl-l-leucine (PubChem CID 94418); ipase (PubChem SID 417478); l-tyrosine (PubChem CID 6057); sodium borate (PubChem CID 122402923); substrate for angiotensin (PubChem CID 569657); tyrosinase from mushroom (PubChem SID 318693930); α-amylase (PubChem SID 318693948).