Adhaeribacter terrigena sp. nov., Isolated from Korean Soil

Abstract

A bacterial strain, designated BT258^T, was isolated from a soil sample collected from Uijeongbu-si, Gyeong-do Province, Republic of Korea. Cells were Gram stain negative, aerobic, rod shaped, motile by gliding, and formed light pink-pigmented colonies on agar plates. Growth of the isolate was observed at 10-37 °C and pH 6-7. A 16S rRNA gene sequence analysis revealed that strain BT258^T is a member of the genus Adhaeribacter in the family Hymenobacteraceae and had the highest sequence similarity with 'Adhaeribacter soli' MA2^T (97.1%), Adhaeribacter terreus DNG6^T (96.6%), and Adhaeribacter terrae HY02^T (96.5%). The predominant respiratory quinone of the isolate was MK-7, the main polar lipid was phosphatidylethanolamine, and the major fatty acids were C_15:0 iso (37.7%), summed feature 4 (C_17:1 anteiso B/iso-C_17:1 I; 16.8%), and C_16:0 (10.3%). The draft genome of strain BT258^T had a whole length of 4,974,022 bp and DNA G + C content of 46.0 mol%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the novel isolate and 'Adhaeribacter soli' and seven other Adhaeribacter species ranged from 17.9 to 22.7% and 69.7 to 77.9%, respectively. On the basis of its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain BT258^T represents a novel species in the genus Adhaeribacter, for which the name Adhaeribacter terrigena sp. nov. is proposed. The type strain is BT258^T (= KCTC 72409 ^T = JCM 34303 ^T).