Inactivation of SARS-CoV-2 isolates from lineages B.1.1.7 (Alpha), P.1 (Gamma) and B.1.110 by heating and UV irradiation

S Ulloa; C Bravo; E Ramirez; R Fasce; J Fernandez

doi:10.1016/j.jviromet.2021.114216

Inactivation of SARS-CoV-2 isolates from lineages B.1.1.7 (Alpha), P.1 (Gamma) and B.1.110 by heating and UV irradiation

J Virol Methods. 2021 Sep:295:114216. doi: 10.1016/j.jviromet.2021.114216. Epub 2021 Jun 22.

Authors

S Ulloa¹, C Bravo¹, E Ramirez², R Fasce², J Fernandez³

Affiliations

¹ Molecular Genetics Sub Department, Institute of Public Health of Chile, Santiago, Chile.
² Viral Diseases Sub Department, Institute of Public Health of Chile, Santiago, Chile.
³ Molecular Genetics Sub Department, Institute of Public Health of Chile, Santiago, Chile. Electronic address: jfernand@ispch.cl.

Abstract

Currently, the rapid global spread of SARS-CoV-2 is related to G clade (including GH, GR, GRY and GV clades), which are associated with more than 98 % of sequenced viral isolates worldwide. The unprecedented velocity of spread of SARS-CoV-2 outbreak represents a critical need for prevention strategies. Vaccines are recently being available and antiviral drugs have shown limited efficacy in COVID-19 patients. Thus, it is needed to know how to reduce the infectivity of the virus by different physicochemical conditions in order to prevent exposure to contaminated material. This work describes heating and irradiating UV-C light procedures to reduce the infectivity of SARS-CoV-2 belonging to different three lineages. Results of physicochemical treatment showed no differences among viral lineages. Analytical conditions for efficient inactivation of SARS-CoV-2 were determined.

Keywords: Alpha; COVID-19; Coronavirus; Gamma; UV dosage; UV-C; Virus inactivation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19 / virology
Hot Temperature
Humans
SARS-CoV-2 / genetics
SARS-CoV-2 / isolation & purification
SARS-CoV-2 / radiation effects*
Ultraviolet Rays
Virus Inactivation / radiation effects*