Sex- and tissue-dependent creatine uptake in response to different creatine monohydrate doses in male and female Sprague-Dawley rats

Tyler C Dunham; Jensen E Murphy; Rebecca E K MacPherson; Val Andrew Fajardo; Wendy E Ward; Brian D Roy

doi:10.1139/apnm-2021-0301

Sex- and tissue-dependent creatine uptake in response to different creatine monohydrate doses in male and female Sprague-Dawley rats

Appl Physiol Nutr Metab. 2021 Oct;46(10):1298-1302. doi: 10.1139/apnm-2021-0301. Epub 2021 Jun 25.

Authors

Tyler C Dunham¹, Jensen E Murphy¹, Rebecca E K MacPherson², Val Andrew Fajardo¹, Wendy E Ward¹, Brian D Roy¹

Affiliations

¹ Department of Kinesiology, Brock University, St. Catharines, ON, Canada.
² Department of Health Sciences, Brock University, St. Catharines, ON, Canada.

PMID: 34171201
DOI: 10.1139/apnm-2021-0301

Abstract

Sprague-Dawley rats (n = 32) underwent 8-weeks of creatine monohydrate (CM) supplementation (0, 2.5, 5, and 10 g/L). Total creatine (TCr) concentrations in female white fibre-dominant gastrocnemius (WGAS) and cardiac muscle (HRT) were significantly higher compared with males (p < 0.05). CM supplementation increased TCr concentrations in female WGAS (p < 0.05) and HRT (p < 0.01) and in male red fibre-dominant gastrocnemius muscle (RGAS) (p < 0.05). Future research should further investigate sex-differences in basal levels of TCr and the response to CM supplementation. Novelty: There is a sex- and tissue-dependant response to CM supplementation in rats.

Keywords: cardiac and skeletal muscle; creatine; créatine; cytoprotection; différences sexuelles; muscle cardiaque et squelettique; sex-differences.

MeSH terms

Animals
Creatine / administration & dosage
Creatine / pharmacokinetics*
Dietary Supplements*
Female
Male
Muscle Fibers, Skeletal
Muscle Fibers, Slow-Twitch
Muscle, Skeletal / metabolism*
Myocardium / metabolism*
Rats
Rats, Sprague-Dawley
Sex Factors*

Substances

Creatine