Anti-biofilm activity of antibiotic-loaded Hylomate®

Mariana Albano; Kerryl E Greenwood-Quaintance; Melissa J Karau; Jayawant N Mandrekar; Robin Patel

doi:10.1016/j.ijcha.2021.100801

Anti-biofilm activity of antibiotic-loaded Hylomate®

Int J Cardiol Heart Vasc. 2021 Jun 8:34:100801. doi: 10.1016/j.ijcha.2021.100801. eCollection 2021 Jun.

Authors

Mariana Albano¹, Kerryl E Greenwood-Quaintance¹, Melissa J Karau¹, Jayawant N Mandrekar², Robin Patel^{1

3}

Affiliations

¹ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States.
² Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, United States.
³ Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN, United States.

Abstract

Introduction: Antibiotic envelopes are being developed for cardiac implantable electronic device (CIED) wrapping to reduce the risk of infections.

Methods: Fifteen CIED infection-associated bacterial isolates of Staphylococcus aureus, Staphylococcus epidermidis and Cutibacterium acnes were used to assess in vitro biofilm formation on Hylomate® compared to titanium, silicone and polyurethane coupons pre-treated with vancomycin (400 µg/ml), bacitracin (1000 U/ml) or a combination of rifampin (80 µg/ml) plus minocycline (50 µg/ml). Scanning electron microscopy (SEM) was performed to visualize bacteria on Hylomate®.

Results: There was significantly less (p < 0.05) S. aureus and S. epidermidis on Hylomate® pre-treated with vancomycin, bacitracin or rifampin plus minocycline after 24 h of incubation (≤1.00 log₁₀ CFU/cm²) compared with titanium, silicone or polyurethane pre-treated with vancomycin, bacitracin or rifampin plus minocycline. C. acnes biofilms were not detected (≤1.00 log₁₀ CFU/cm²) on pre-treated Hylomate® coupons.

Conclusions: This study showed that Hylomate® coupons pre-treated with antibiotics reduced staphylococcal and C. acnes biofilm formation in vitro.

Keywords: Antibacterial envelope; Biofilm; Cardiac device infection; Cutibacterium acnes; Staphylococcus aureus; Staphylococcus epidermidis.