Highly selective two-photon fluorescent off-on probes for imaging tyrosinase activity in living cells and tissues

Yixin Cui; Sang Jun Park; Xiaofeng Wu; Rui Wang; Sujie Qi; Hwan Myung Kim; Juyoung Yoon

doi:10.1039/d1cc02374h

Highly selective two-photon fluorescent off-on probes for imaging tyrosinase activity in living cells and tissues

Chem Commun (Camb). 2021 Jul 13;57(56):6911-6914. doi: 10.1039/d1cc02374h.

Authors

Yixin Cui¹, Sang Jun Park², Xiaofeng Wu¹, Rui Wang¹, Sujie Qi¹, Hwan Myung Kim², Juyoung Yoon¹

Affiliations

¹ Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 120-750, Korea. jyoon@ewha.ac.kr.
² Department of Chemistry and Department of Energy Systems Research, Ajou University, Suwon, 443-749, Korea. kimhm@ajou.ac.kr.

PMID: 34152336
DOI: 10.1039/d1cc02374h

Abstract

A coumarin-based two-photon (TP) fluorescent off-on probe has been developed for detecting tyrosinase activity. High selectivity, sensitivity and biocompatibility enable the probes to successfully image tyrosinase activity in live cells and tissues using TP microscopy.

MeSH terms

Animals
Cell Line, Tumor
Coumarins / chemistry*
Coumarins / metabolism
Drug Design
Fluorescent Dyes / chemistry*
Fluorescent Dyes / metabolism
Humans
Limit of Detection
Mice
Microscopy, Fluorescence, Multiphoton
Monophenol Monooxygenase / analysis*
Monophenol Monooxygenase / metabolism
Photons
Rats

Substances

Coumarins
Fluorescent Dyes
Monophenol Monooxygenase