Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains

Cecilia Krampah; Franklin Nyanzu; Abraham Quaye; Patrick Adu; Emmanuel Akomanin Asiamah; Benjamin Aboagye; David Larbi Simpong

doi:10.1016/j.plabm.2021.e00239

Crude extracts from Allium cepa skin and Sorghum bicolor seed can provide as non-toxic and eco-friendly cytoplasmic stains

Pract Lab Med. 2021 Jun 5:26:e00239. doi: 10.1016/j.plabm.2021.e00239. eCollection 2021 Aug.

Authors

Cecilia Krampah¹, Franklin Nyanzu¹, Abraham Quaye¹, Patrick Adu², Emmanuel Akomanin Asiamah³, Benjamin Aboagye⁴, David Larbi Simpong²

Affiliations

¹ Department of Medical Laboratory Science, School of Allied Health Sciences, University of Ghana, Ghana.
² Department of Medical Laboratory Science, School of Allied Health Sciences, University of Cape Coast, Ghana.
³ Department of Medical Laboratory Science, University of Allied Health Science, Ghana.
⁴ Forensic Science Department, University of Cape Coast, Ghana.

Abstract

Introduction: Staining is an important histological process; however, the use of non-toxic and environmentally friendly products is generally required. We explored the staining quality of two natural plants, Allium cepa skin and Sorghum bicolor seed extract on the cytoplasm.

Materials and methods: Distilled water at 37 °C and 1% acid-ethanol were respectively used to extract the dyes from Allium cepa skin and Sorghum bicolor seed.

Result: The application of these two dyes on rodent tissue showed an excellent cytoplasmic histomorphology.

Conclusion: Allium cepa skin and Sorghum bicolor seed extracts are good cytoplasmic dyes when used as counterstain for haematoxylin.

Keywords: Cell; Cytoplasm; Extract; Natural dye; Nucleus; Stain.