Modeling colorectal cancers using multidimensional organoids

Ibrahim M Sayed; Amer Ali Abd El-Hafeez; Priti P Maity; Soumita Das; Pradipta Ghosh

doi:10.1016/bs.acr.2021.02.005

Modeling colorectal cancers using multidimensional organoids

Adv Cancer Res. 2021:151:345-383. doi: 10.1016/bs.acr.2021.02.005. Epub 2021 Mar 26.

Authors

Ibrahim M Sayed¹, Amer Ali Abd El-Hafeez², Priti P Maity², Soumita Das³, Pradipta Ghosh⁴

Affiliations

¹ Department of Pathology, University of California, San Diego, CA, United States.
² Department of Cellular and Molecular Medicine, University of California, San Diego, CA, United States.
³ Department of Pathology, University of California, San Diego, CA, United States; Rebecca and John Moore Comprehensive Cancer Center, University of California, San Diego, CA, United States; HUMANOID Center of Research Excellence (CoRE), University of California, San Diego, CA, United States. Electronic address: sodas@health.ucsd.edu.
⁴ Department of Cellular and Molecular Medicine, University of California, San Diego, CA, United States; Rebecca and John Moore Comprehensive Cancer Center, University of California, San Diego, CA, United States; Department of Medicine, University of California, San Diego, CA, United States; Veterans Affairs Medical Center, San Diego, CA, United States; HUMANOID Center of Research Excellence (CoRE), University of California, San Diego, CA, United States. Electronic address: prghosh@health.ucsd.edu.

Abstract

Organoids have revolutionized cancer research as highly adaptable models that enable an array of experimental techniques to interrogate tissue morphology and function. Because they preserve the genetic, phenotypic, and behavioral traits of their source tissue, organoids have gained traction as the most relevant models for drug discovery, tracking therapeutic response and for personalized medicine. As organoids are indisputably becoming a mainstay of cancer research, this review specifically addresses how colon-derived organoids can be perfected as multidimensional, scalable, reproducible models of healthy, pre-neoplastic and neoplastic conditions of the colon and for use in high-throughput "Phase-0" human clinical trials-in-a-dish.

Keywords: CRC; Clinical trials; Coculture; Colon organoid; Drug screening; Multidimensional.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Colorectal Neoplasms / pathology*
Humans
Models, Biological*
Organoids / pathology*
Precision Medicine / methods
Precision Medicine / trends
Primary Cell Culture / methods
Primary Cell Culture / trends
Tissue Culture Techniques / methods*
Tissue Culture Techniques / trends

Abstract

Publication types

MeSH terms

Grants and funding