Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting

Hongdou Liu; Xuhong Ye; Songling Chen; Aobo Sun; Xinying Duan; Yanqing Zhang; Hongtao Zou; Yulong Zhang

doi:10.1016/j.scitotenv.2021.148381

Chitosan as additive affects the bacterial community, accelerates the removals of antibiotics and related resistance genes during chicken manure composting

Sci Total Environ. 2021 Oct 20:792:148381. doi: 10.1016/j.scitotenv.2021.148381. Epub 2021 Jun 10.

Authors

Hongdou Liu¹, Xuhong Ye¹, Songling Chen¹, Aobo Sun¹, Xinying Duan¹, Yanqing Zhang², Hongtao Zou³, Yulong Zhang¹

Affiliations

¹ College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, China; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shenyang 110866, Liaoning, China; Key Laboratory of Arable Land Conservation (Northeast China), Ministry of Agriculture and Rural Areas, Shenyang 110866, Liaoning, China.
² College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, China; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shenyang 110866, Liaoning, China; Key Laboratory of Arable Land Conservation (Northeast China), Ministry of Agriculture and Rural Areas, Shenyang 110866, Liaoning, China; Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agricultural Sciences, China.
³ College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, China; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shenyang 110866, Liaoning, China; Key Laboratory of Arable Land Conservation (Northeast China), Ministry of Agriculture and Rural Areas, Shenyang 110866, Liaoning, China. Electronic address: hongtaozou208@126.com.

PMID: 34146805
DOI: 10.1016/j.scitotenv.2021.148381

Abstract

Manures, storages for antibiotic resistance genes (ARGs), pollute soil and water as well as endanger human health. Recently, we have been searching a better solution to remove antibiotics and ARGs during aerobic composting. Here, the dynamics of chitosan addition on the profiles of 71 ARGs, bacterial communities, chlortetracycline (CTC), ofloxacin (OFX) were investigated in chicken manure composting and compared with zeolite addition. Chitosan addition effectively reduces antibiotics contents (CTC under detection limit, OFX 90.96%), amounts (18) and abundance (56.7%, 11.1% higher than zeolite addition) of ARGs and mobile genetic elements (MGEs) after 42 days composting. Network analysis indicated that a total of 27 genera strains assigned into 4 phyla (Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes) were the potential hosts of ARGs. Redundancy analysis (RDA) demonstrated that bacterial community succession is the main contributor in the variation of ARGs. Overall, chitosan addition may effect bacterial composition by influencing physic-chemical properties and the concentration of antibiotics, Cu²⁺, Zn²⁺ to reduce the risk of ARG transmission. This study gives a new strategy about antibiotics and ARGs removal from composting on the basis of previous studies.

Keywords: Aerobic composting; Antibiotic resistance gene (ARG); Chitosan addition; In-feed antibiotic; Potential host bacteria.

MeSH terms

Animals
Anti-Bacterial Agents
Chickens
Chitosan*
Composting*
Genes, Bacterial
Humans
Manure

Substances

Anti-Bacterial Agents
Manure
Chitosan