Antioxidant balance in plasma of patients on home parenteral nutrition: A pilot study comparing three different lipid emulsions

Abstract

Background & aims: Parenteral nutrition (PN) can supply all essential nutrients to a patient with gastrointestinal insufficiency. However, the sensitivity to lipid peroxidation might increase in those receiving PN, especially home parenteral nutrition (HPN). This study aimed to investigate whether PN affects the antioxidant balance of plasma of HPN patients without comorbidities and whether this balance is influenced by comorbidities and according to the type of lipid emulsion included in the PN.

Methods: Adult patients on HPN (n = 86) received one of three types of lipid emulsion (based on 1) soyabean oil, 2) olive and soyabean oil or 3) soyabean, coconut, olive and fish oil) in all-in-one mixtures; in addition healthy controls (n = 66) were studied as comparators. HPN patients were classified to the following subgroups: 1) patients without (n = 58) or with (n = 28) comorbidities 2) patients on Intralipid (G_INTRA, n = 53), ClinOleic (G_CLIN, n = 17) or SMOFlipid (G_SMOFn = 16). The activities of total glutathione peroxidase (GSH-Px), selenium dependent glutathione peroxidase (Se-GSHPx) and glutathione S-transferase (GST) in plasma were determined spectrophotometrically. The antioxidant potential of plasma was determined using oxygen radical absorbance capacity (ORAC). The lipid peroxidation marker malondialdehyde (MDA) was analyzed with high performance liquid chromatography.

Results: MDA concentration was the highest in G_INTRA and the lowest in G_SMOF (p < 0.05). G_SMOF also had the highest activity of GSH-Px. No differences in Se-GSHPx, GST and ORAC were observed among G_INTRA, G_CLIN and G_SMOF. Comparing with healthy controls, significantly lower GST (p = 0.0293) and ORAC (p < 0.0001) were observed in the HPN patients. Among all measured parameters only the concentration of MDA was significantly higher in patients with comorbidities compared to those without them. Comorbidities did not influence MDA level in G_INTRA and G_SMOF being still the lowest in G_SMOF (p = 0.0033). In contrast, significantly higher MDA level was observed for G_CLIN in those with vs. without comorbidities (p = 0.0262).

Conclusions: Patients on HPN have lower antioxidant defenses than healthy controls. The type of lipid emulsion used in HPN affects lipid peroxidation (even after taking into account comorbidities which often involve oxidative stress) being the highest in G_INTRA and the lowest in G_SMOF. Thus, to minimize the risk of oxidative stress, SMOFlipid can be considered in patients in HPN especially for those with comorbidities. ClinOleic can be considered in HPN patients without comorbidities. The observation should be confirmed in larger studies.

Keywords: Glutathione peroxidase; Lipid emulsions; Malondialdehyde; ORAC; Oxidative stress; Parenteral nutrition.