CTPS forms the cytoophidium in zebrafish

Chia-Chun Chang; Gerson Dierley Keppeke; Christopher L Antos; Min Peng; Luis Eduardo Coelho Andrade; Li-Ying Sung; Ji-Long Liu

doi:10.1016/j.yexcr.2021.112684

CTPS forms the cytoophidium in zebrafish

Exp Cell Res. 2021 Aug 15;405(2):112684. doi: 10.1016/j.yexcr.2021.112684. Epub 2021 Jun 12.

Authors

Chia-Chun Chang¹, Gerson Dierley Keppeke², Christopher L Antos¹, Min Peng³, Luis Eduardo Coelho Andrade⁴, Li-Ying Sung⁵, Ji-Long Liu⁶

Affiliations

¹ School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
² School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP 04023-062, Brazil.
³ Institute of Biotechnology, National Taiwan University, Taipei, 106, Taiwan.
⁴ Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP 04023-062, Brazil.
⁵ Institute of Biotechnology, National Taiwan University, Taipei, 106, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan.
⁶ School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, United Kingdom. Electronic address: jilong.liu@dpag.ox.ac.uk.

PMID: 34129847
DOI: 10.1016/j.yexcr.2021.112684

Abstract

Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-l-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates.

Keywords: CTPS; Cytoophidium; Zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon-Nitrogen Ligases / metabolism*
Cell Line
Cytidine Triphosphate / metabolism*
Eukaryota / cytology*
Nitric Oxide Synthase / metabolism
Prokaryotic Cells / cytology*
Zebrafish

Substances

Cytidine Triphosphate
Nitric Oxide Synthase
Carbon-Nitrogen Ligases

Abstract

Publication types

MeSH terms

Substances

Grants and funding