Moxibustion Protects Dopaminergic Neurons in Parkinson's Disease through Antiferroptosis

Zifeng Huang; Wenwen Si; Xinrong Li; Shanyu Ye; Xuelei Liu; Yichun Ji; Xiaoqian Hao; Dongfeng Chen; Meiling Zhu

doi:10.1155/2021/6668249

Moxibustion Protects Dopaminergic Neurons in Parkinson's Disease through Antiferroptosis

Evid Based Complement Alternat Med. 2021 Apr 16:2021:6668249. doi: 10.1155/2021/6668249. eCollection 2021.

Authors

Zifeng Huang^{1

2}, Wenwen Si¹, Xinrong Li^{1

2}, Shanyu Ye³, Xuelei Liu¹, Yichun Ji⁴, Xiaoqian Hao^{1

2}, Dongfeng Chen³, Meiling Zhu¹

Affiliations

¹ Traditional Chinese Medicine Innovation Research Center, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Shenzhen, Guangdong 518104, China.
² Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China.
³ Department of Anatomy, The Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
⁴ Shenzhen Bao An Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China.

Abstract

Ferroptosis is associated with neural degeneration of dopaminergic neurons in Parkinson's disease (PD). However, how to control the level of ferroptosis in PD remains unclear. Clinically, moxibustion has been used to treat PD and has an apparent therapeutic effect on improving the motor symptoms of PD. In the present study, the PD rat model was constructed by two-point stereotactic 6-hydroxydopamine injection. Then, moxibustion was used to treat the PD rats. The expression of glutathione peroxidase 4 (GPX4) and Ferritin Heavy Chain 1 (FTH1), the level of reactive oxygen species (ROS), and the morphology of mitochondrial were detected to evaluate the level of ferroptosis. The results showed that moxibustion treatment of Shi's moxa sticks could reduce the behavioral score, alleviate the level of ferroptosis, decrease mitochondrial damage, and improve dopaminergic neuron survival. In conclusion, the present study results indicated that Shi's moxa sticks could effectively suppress the level of ferroptosis, thereby improving the survival of dopaminergic neurons in the SNpc of PD rats, which may provide a promising complementary and alternative therapy for PD patients.