Biochar application to rice with 15N-labelled fertilizers, enhanced leaf nitrogen concentration and assimilation by improving morpho-physiological traits and soil quality

Saif Ullah; Quan Zhao; Ke Wu; Izhar Ali; He Liang; Anas Iqbal; Shanqing Wei; Fangwei Cheng; Shakeel Ahmad; Ligeng Jiang; Syeda Wajeeha Gillani; Amanullah; Shazma Anwar; Zaid Khan

doi:10.1016/j.sjbs.2021.03.003

Biochar application to rice with ¹⁵N-labelled fertilizers, enhanced leaf nitrogen concentration and assimilation by improving morpho-physiological traits and soil quality

Saudi J Biol Sci. 2021 Jun;28(6):3399-3413. doi: 10.1016/j.sjbs.2021.03.003. Epub 2021 Mar 14.

Authors

Affiliations

¹ Key Laboratory of Crop Cultivation and Farming System, Guangxi University, Nanning 530004, China.
² State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China.
³ Department of Agronomy, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.

Abstract

Leaf nitrogen (N) concentration plays an important role in biochemical and physiological functions, and N availability directly influences rice yield. However, excessive N fertilization is considered to be a root cause of environmental issues and low nitrogen use efficiency. Therefore, the selection of appropriate nutrient management practices and organic amendments is key to maximizing nitrogen uptake and maintaining high and sustainable rice production. Here, we evaluated the effects of different ¹⁵N-labelled nitrogen sources (urea, ammonium nitrate, and ammonium sulfate at 315 kg ha^-1) with or without biochar (30 t ha^-1) on paddy soil properties, root growth, leaf gas exchange, N metabolism enzymes, and N uptake in the early and late seasons of 2019. We found significant differences among N fertilizer sources applied with or without biochar (P < 0.05). Across the seasons, the combination of biochar with N fertilizers significantly increased soil organic carbon by 51.21% and nitrogen availability by 27.51% compared with N fertilizers alone. Correlation analysis showed that rice root morphological traits were strongly related to soil chemical properties, and higher root growth was measured in the biochar treatments. Similarly, net leaf photosynthetic rate averaged 9.34% higher, chlorophyll (Chl) a concentration 12.91% higher, and Chl b concentration 10.05% higher in the biochar treatments than in the biochar-free treatments across the seasons. Notably, leaf ¹⁵N concentration was 23.19% higher in the biochar treatments in both seasons. These results illustrated higher activities of N metabolism enzymes such as NR, GS, and GOGAT by an average 23.44%, 11.26% and 18.16% in the biochar treatments across the seasons, respectively. The addition of biochar with synthetic N fertilizers is an ecological nutrient management strategy that can increase N uptake and assimilation by ameliorating soil properties and improving the morpho-physiological factors of rice.

Keywords: Biochar; Nitrogen concentration; Photosynthetic pigments; Rice; Root morphology.