Enzyme-free detection of hydrogen peroxide with a hybrid transducing system based on sodium carboxymethyl cellulose, poly(3,4-ethylenedioxythiophene) and prussian blue nanoparticles

Sinan Uzuncar; Nizamettin Ozdogan; Metin Ak

doi:10.1016/j.aca.2021.338664

Enzyme-free detection of hydrogen peroxide with a hybrid transducing system based on sodium carboxymethyl cellulose, poly(3,4-ethylenedioxythiophene) and prussian blue nanoparticles

Anal Chim Acta. 2021 Aug 8:1172:338664. doi: 10.1016/j.aca.2021.338664. Epub 2021 May 24.

Authors

Sinan Uzuncar¹, Nizamettin Ozdogan², Metin Ak³

Affiliations

¹ Environmental Engineering Department, Engineering Faculty, Zonguldak Bülent Ecevit University, 67100, Zonguldak, Turkey.
² Environmental Engineering Department, Engineering Faculty, Zonguldak Bülent Ecevit University, 67100, Zonguldak, Turkey. Electronic address: nozdogan@beun.edu.tr.
³ Chemistry Department, Faculty of Art and Science, Pamukkale University, 20070, Denizli, Turkey. Electronic address: metinak@pau.edu.tr.

PMID: 34119021
DOI: 10.1016/j.aca.2021.338664

Abstract

Herein, we report a two-layered hybrid catalytic interface composed of carboxymethyl cellulose (CMC), poly (3,4-ethylene dioxythiophene) (PEDOT), Prussian blue (PB) nanoparticles and Nickel-Hexacyanoferrate (Ni-HCF) layer for the enzyme-free detection of hydrogen peroxide (H₂O₂). Whereas the first layer, CMC:PEDOT:PB, is responsible for generating amperometric signals toward H₂O₂, Ni-HCF on CMC:PEDOT:PB layer is playing an active role as an operational stability-enhancer. In the study, where the systematic optimization of the sensor electrode is presented using cyclic voltammetry (CV), amperometry and electrochemical impedance spectroscopy (EIS) technique, the physical and chemical properties of the hybrid composite systems constructed is also supported by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) techniques. The amperometric signal generation of the H₂O₂ sensor was linear between 1 and 100 μM (R² = 0.999) with a sensitivity of 416.11 μA mM^-1cm^-2, providing a limit of detection (LOD) of 0.33 μM. The sensing system, which was not affected by the various interfering molecules, creates a successful sensor platform for H₂O₂ measurements in tap water with a high recovery value between 94.0% and 110.5% and relatively small RSD in the range of 0.4-5.2%.

Keywords: Cellulose; Hybrid; Nickel hexacyanoferrate; Poly(3,4-ethylenedioxythiophene); Prussian blue.