Identification and characterization of andalusicin: N-terminally dimethylated class III lantibiotic from Bacillus thuringiensis sv. andalousiensis

Anastasiia Grigoreva; Julia Andreeva; Dmitry Bikmetov; Anastasiia Rusanova; Marina Serebryakova; Andrea Hernandez Garcia; Darya Slonova; Satish K Nair; Guy Lippens; Konstantin Severinov; Svetlana Dubiley

doi:10.1016/j.isci.2021.102480

Identification and characterization of andalusicin: N-terminally dimethylated class III lantibiotic from Bacillus thuringiensis sv. andalousiensis

iScience. 2021 Apr 29;24(5):102480. doi: 10.1016/j.isci.2021.102480. eCollection 2021 May 21.

Authors

Anastasiia Grigoreva^{1

2}, Julia Andreeva^{1

2}, Dmitry Bikmetov^{3

4}, Anastasiia Rusanova², Marina Serebryakova^{2

5}, Andrea Hernandez Garcia⁶, Darya Slonova¹, Satish K Nair⁶, Guy Lippens⁷, Konstantin Severinov^{1

3

4

8}, Svetlana Dubiley^{1

2}

Affiliations

¹ Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia.
² Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
³ Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
⁴ Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia.
⁵ A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
⁶ Department of Biochemistry, University of Illinois at Urbana-Champaign, Champaign, IL 61801 USA.
⁷ Toulouse Biotechnology Institute (TBI), Université de Toulouse, CNRS, INRA, INSA, Toulouse 31077, France.
⁸ Waksman Institute for Microbiology, Piscataway, NJ 08854-8020, USA.

Abstract

Lanthipeptides, ribosomally synthesized and post-translationally modified peptides (RiPPs), can be divided into five classes based on their structures and biosynthetic pathways. Class I and II lanthipeptides have been well characterized, whereas less is known about members of the other three classes. Here, we describe a new family of class III lanthipeptides from Firmicutes. Members of the family are distinguished by the presence of a single carboxy-terminal labionin. We identified and characterized andalusicin, a representative of this family. Andalusicin bears two methyl groups at the α-amino terminus, a post-translational modification that has not previously been identified in class III lanthipeptides. Mature andalusicin A shows bioactivity against various Gram-positive bacteria, an activity that is highly dependent on the α-N dimethylation.

Keywords: Biochemistry; Biosynthesis; Chemistry; Medical biochemistry.

Grants and funding

R01 GM079038/GM/NIGMS NIH HHS/United States