Cancer cell lines are an indispensable tool in the cancer research. Since the first human cell line, HeLa was established in the 1950s, thousands of cancer cell lines have been established, including 637 characterized leukemia-lymphoma cell lines. The probability to successfully establish cancer cell lines is a low by traditional methods, and the addition of regulatory factors is often required. However, a novel "conditional reprogramming" technology can improve this situction. The establishment and description of a new cell line should be consistent with international guidelines. Cancer cell lines are mainly used in the research of tumor pathogenesis and drug development. Scientists have developed many kinds of cell line panels which can be used for the high-throughput screening of anticancer drugs. Mycoplasma contamination and/or cross-contamination from other cells should be avoided during the use of cell lines. The establishment of a cell model passport database can prevent those misidentifications. In this review, the types, establishment and usage of leukemia-lymphoma cell lines as well as points of attention when using them are summarized briefly.
题目: 白血病-淋巴瘤细胞系的建立与使用.
摘要: 癌细胞系是肿瘤研究中必不可少的工具，自20世纪50年代第一个人类细胞系HeLa建立以来，至今已经有数千种癌细胞系，其中特征性的白血病-淋巴瘤细胞系有637个。传统癌细胞系的建立成功率低，常需要加入调节因子，”条件性重编程”技术可提高成功率。每个新细胞系建立及其表征的描述需符合指南要求。肿瘤细胞系主要用于肿瘤发病机制的研究和药物的开发，科学家已经开发出多种细胞系的组合用于抗癌药的高通量筛选。使用细胞系时，需注意支原体污染和/或来自其他细胞的交叉污染，建立细胞模型护照数据库可防止错误识别。本文就白血病-淋巴瘤细胞系的种类、建立、使用以及使用时的注意事项等作一综述.