The overproduction of reactive oxygen species during abiotic stress in plants causes oxidative stress that damage the cell normal functions. For reactive oxygen species (ROS) scavenging, plants developed a defense system with antioxidant enzymes. To measure the oxidative stress and antioxidant enzymes activity spectral enzymatic analysis was used, that is material-intensive, time-consuming, and inefficient. In the present study, the four more studied and main antioxidant enzymatic assays are miniaturized in a 96-well plate system and monitored the activity of enzymes by spectrophotometry. This method has obvious advantages over the standard cuvette analysis method because the miniaturization of the 96-well microplate system decreases the amount of reaction mixture and enzymes extract, thrifts working time, and consumable costs as well.
Keywords: 96-well microplate; Enzymatic activity; Oxidative stress; Spectrophotometry.