Nanoparticles are an extensive class of naturally occurred or man-made objects; it has been widely using in our daily life. As increasing usage, nanoparticles are also released into the environment and are becoming an emerged environmental pollution. In the past decade, impact of nanoparticles on plant growth and development has been becoming a major research topic in the environmental toxicology. In this chapter, we introduce a step-by-step protocol for investigating the effects of nanoparticles on plant growth and development as well as biomass production. Additionally, this protocol also tests the water content and the rate of root and up-ground part to better explain the impact of nanoparticles on plant growth. This protocol adopts plant tissue culture technology to culture plants which makes test easier and can be tested anytime during the year.
Keywords: Nanoparticle; Plant; Plant biomass; Root development; Seed germination.