Characterizing brain metabolic function ex vivo with acute mouse slice punches

Guoyuan Qi; Yashi Mi; Fei Yin

doi:10.1016/j.xpro.2021.100559

Characterizing brain metabolic function ex vivo with acute mouse slice punches

STAR Protoc. 2021 May 23;2(2):100559. doi: 10.1016/j.xpro.2021.100559. eCollection 2021 Jun 18.

Authors

Guoyuan Qi¹, Yashi Mi¹, Fei Yin^{1

2

3}

Affiliations

¹ Center for Innovation in Brain Science, University of Arizona Health Sciences, University of Arizona, 1230 N Cherry Ave, Tucson, AZ 85724, USA.
² Department of Pharmacology, College of Medicine Tucson, University of Arizona, Tucson, AZ 85724, USA.
³ Graduate Interdisciplinary Program in Neuroscience, University of Arizona, Tucson, AZ 85721, USA.

Abstract

Mitochondrial dysfunction and metabolic reprogramming are implicated in a variety of neurological disorders. Here, we present a protocol that enables complex profiling of brain metabolic function using acute mouse brain slices ex vivo. Utilizing differential metabolic conditions, substrates, and inhibitors, this protocol can be broadly applied to determine metabolic shift or reprogramming upon genetic manipulations, pathological insults, or therapeutic interventions and could thus further the understanding of the dynamic role of energy metabolism in brain physiological function and diseases. For complete details on the use and execution of this protocol, please refer to Qi et al. (2021).

Keywords: Metabolism; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism*
Energy Metabolism
Glycolysis
Mice
Reproducibility of Results

Grants and funding

RF1 AG068175/AG/NIA NIH HHS/United States