Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues

Ruili Feng; Dongyue Wang; Yanlu Chen; Jing Lu; Liang Gao

doi:10.1016/j.xpro.2021.100546

Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues

STAR Protoc. 2021 May 19;2(2):100546. doi: 10.1016/j.xpro.2021.100546. eCollection 2021 Jun 18.

Authors

Ruili Feng^{1

2

3}, Dongyue Wang^{1

2

3}, Yanlu Chen^{1

2

3}, Jing Lu^{1

2

3}, Liang Gao^{1

2

3}

Affiliations

¹ Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.
² Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China.
³ Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024, China.

Abstract

Here, we describe a protocol to construct, calibrate, and operate a versatile tiling light sheet microscope for imaging cleared tissues. The microscope uses adjustable tiling light sheets to achieve higher spatial resolution and better optical sectioning ability than conventional light sheet microscopes and to image cleared tissues with the cellular to the subcellular spatial resolution. It is compatible with all tissue clearing methods and aligned semiautomatically through the phase modulation of the illumination light. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020).

Keywords: Microscopy; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / diagnostic imaging
Equipment Design
Image Processing, Computer-Assisted / methods*
Mice
Microscopy, Fluorescence* / instrumentation
Microscopy, Fluorescence* / methods