RNA sequencing data for gamma radiation response in the extremotolerant tardigrade Ramazzottius varieornatus

Yuki Yoshida; Daiki D Horikawa; Tetsuya Sakashita; Yuichiro Yokota; Yasuhiko Kobayashi; Masaru Tomita; Kazuharu Arakawa

doi:10.1016/j.dib.2021.107111

RNA sequencing data for gamma radiation response in the extremotolerant tardigrade Ramazzottius varieornatus

Data Brief. 2021 May 9:36:107111. doi: 10.1016/j.dib.2021.107111. eCollection 2021 Jun.

Authors

Yuki Yoshida^{1

2}, Daiki D Horikawa^{1

2}, Tetsuya Sakashita³, Yuichiro Yokota³, Yasuhiko Kobayashi³, Masaru Tomita^{1

2

4}, Kazuharu Arakawa^{1

2

4}

Affiliations

¹ Keio University, Institute for Advanced Biosciences, Tsuruoka, Yamagata 997-0035, Japan.
² Keio University, Graduate School of Media and Governance, Systems Biology Program, Fujisawa, Kanagawa 252-0882, Japan.
³ National Institutes for Quantum and Radiological Science and Technology, Takasaki Advanced Radiation Research Institute, Takasaki, Gunma 370-1292, Japan.
⁴ Keio University, Faculty of Environment and Information Studies, Fujisawa, Kanagawa 252-0882, Japan.

Abstract

Tardigrades are microscopic animals of which terrestrial species are capable of tolerating extreme environments by entering a desiccated ametabolic state known as anhydrobiosis. Intriguingly, they survive high dosage gamma rays (>4,000 Gy), possibly through a mechanism known as cross-tolerance. We hypothesized that anhydrobiosis genes are also regulated during cross-tolerance, thus we submitted Ramazzottius varieornatus to 500 Gy ⁶⁰Co gamma-ray and conducted time-course low-input RNA-Seq. The gene expression was quantified with RSEM and differential expression was determined with DEseq2. Differentially expressed genes were submitted to gene ontology enrichment analysis with GOStat. The transcriptome dynamically shifted nine hours post-exposure.

Keywords: Extremotolerant; Gamma ray; Ramazzottius varieornatus; Transcriptome sequencing.