Overexpression of LINC00673 Promotes the Proliferation of Cervical Cancer Cells

Sheng-Kai Huang; Ruo-Xuan Ni; Wen-Jie Wang; Di Wang; Mei Zhao; Cheng-Zhi Lei; Xiao-Jie Sun; Chang-Zhi Huang; Ping Bai; Yi-Qun Che; Jian-Ping Xu

doi:10.3389/fonc.2021.669739

Overexpression of LINC00673 Promotes the Proliferation of Cervical Cancer Cells

Front Oncol. 2021 May 21:11:669739. doi: 10.3389/fonc.2021.669739. eCollection 2021.

Authors

Sheng-Kai Huang¹, Ruo-Xuan Ni², Wen-Jie Wang^{2

3}, Di Wang¹, Mei Zhao², Cheng-Zhi Lei⁴, Xiao-Jie Sun⁵, Chang-Zhi Huang², Ping Bai⁴, Yi-Qun Che¹, Jian-Ping Xu⁶

Affiliations

¹ Department of Clinical Laboratory, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² Department of Etiology and Carcinogenesis, State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
³ The Center for Disease Control and Prevention of Huai Rou, Beijing, China.
⁴ Department of Gynecologic Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
⁵ Department of Biochemistry, Qiqihar Medical University, Qiqihar, China.
⁶ Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Abstract

Objective: To study the expression of LINC00673 in cervical cancer and cervical intraepithelial neoplasia (CIN) and to explore the role of LINC00673 in the development of cervical cancer.

Methods: The expression of LINC00673 in serum from cervical cancer patients, CIN patients, and healthy participants was detected by RT-qPCR. The function of LINC00673 in cervical cancer cells was analyzed using in vitro and in vivo experiments.

Results: Our results revealed that serum LINC00673 levels were highest in cervical cancer patients, followed by patients with CIN and healthy controls. In vitro experiments demonstrated that overexpression of LINC00673 enhanced the proliferation and cell cycle progression of HeLa and SiHa cells. In vivo experiments showed that the tumor weight and volume of nude mice subcutaneously injected with LINC00673-overexpressing HeLa cells were larger than those of nude mice injected with control cells (P < 0.05). Western blotting showed that cell cycle-related proteins cyclin A2 and cyclin E and interstitial-associated proteins Snail and N-cadherin were upregulated and p53 signaling pathway-related proteins were downregulated in LINC00673-overexpressing HeLa and SiHa cells.

Conclusion: LINC00673 plays an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.

Keywords: LINC00673; cervical cancer; lncRNA; overexpression; proliferation.