Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO2] Show an Impaired C and N Metabolism When Grown at Ambient [CO2]

Abstract

Soybean (Glycine max L.) future response to elevated [CO₂] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO₂]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO₂], were grown in chambers at current and elevated [CO₂] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs were tested through ¹⁵N₂-labeling analysis. Soybeans inoculated with SFJ14-36 strain (isolated at elevated [CO₂]) showed a strong metabolic imbalance, at nodule and leaf levels when grown at ambient [CO₂], probably due to an insufficient supply of N by nodules, as shown by ¹⁵N₂-labeling. In nodules, due to shortage of photoassimilate, C may be diverted to aspartic acid instead of malate in order to improve the efficiency of the C source sustaining N₂-fixation. In leaves, photorespiration and respiration were boosted at ambient [CO₂] in plants inoculated with this strain. Additionally, free phytol, antioxidants, and fatty acid content could be indicate induced senescence due to oxidative stress and lack of nitrogen. Therefore, plants inoculated with Bradyrhizobium strain isolated at elevated [CO₂] may have lost their capacity to form effective symbiosis at ambient [CO₂] and that was translated at whole plant level through metabolic impairment.

Keywords: Bradyrhizobium strains; C and N metabolism; N-fixation; elevated [CO2]; metabolomics; nodule; soybean.