Recent advances in isoelectric focusing of proteins and peptides

Lily Farmerie; Richard R Rustandi; John W Loughney; Mohamed Dawod

doi:10.1016/j.chroma.2021.462274

Recent advances in isoelectric focusing of proteins and peptides

J Chromatogr A. 2021 Aug 16:1651:462274. doi: 10.1016/j.chroma.2021.462274. Epub 2021 May 24.

Authors

Lily Farmerie¹, Richard R Rustandi², John W Loughney², Mohamed Dawod³

Affiliations

¹ Analytical Research & Development, Merck & Co., Inc., Kenilworth, NJ, USA; Pennsylvania State University, College of Engineering, University Park, PA, USA.
² Analytical Research & Development, Merck & Co., Inc., Kenilworth, NJ, USA.
³ Analytical Research & Development, Merck & Co., Inc., Kenilworth, NJ, USA. Electronic address: mohamed.dawod@merck.com.

PMID: 34090060
DOI: 10.1016/j.chroma.2021.462274

Abstract

This review article describes the significant recent advances in Isoelectric Focusing from the period 2015-2020. The review highlights the principles and common challenges faced in Isoelectric Focusing as well as its applications. This review also details the recent advances in various modes of Isoelectric Focusing in various platforms and future directions for the technique.

Keywords: Electrophoresis; Isoelectric focusing; Peptides; Proteins.

Publication types

Review

MeSH terms

Animals
Humans
Isoelectric Focusing / methods*
Peptides / analysis*
Proteins / analysis*

Substances

Peptides
Proteins