Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

Sho Yoshimatsu; Kazuya Edamura; Yumi Yoshii; Aozora Iguchi; Hirotaka Kondo; Hisashi Shibuya; Tsukika Sato; Seiji Shiozawa; Hideyuki Okano

doi:10.1016/j.scr.2021.102375

Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

Stem Cell Res. 2021 May:53:102375. doi: 10.1016/j.scr.2021.102375. Epub 2021 Apr 29.

Authors

Sho Yoshimatsu¹, Kazuya Edamura², Yumi Yoshii², Aozora Iguchi², Hirotaka Kondo³, Hisashi Shibuya³, Tsukika Sato¹, Seiji Shiozawa¹, Hideyuki Okano⁴

Affiliations

¹ Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
² Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
³ Laboratory of Veterinary Pathology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
⁴ Department of Physiology, School of Medicine, Keio University, Tokyo, Japan. Electronic address: hidokano@keio.jp.

PMID: 34088004
DOI: 10.1016/j.scr.2021.102375

Abstract

We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Dogs
Female
Fibroblasts
Induced Pluripotent Stem Cells*
Male
Stem Cell Research
Transgenes