A simple dual-response fluorescent probe for imaging of viscosity and ONOO- through different fluorescence signals in living cells and zebrafish

Chong Wang; Wei Shu; Qianqian Chen; Chunlei Yang; Sa Su; Mengxu Gao; Rubo Zhang; Jing Jing; Xiaoling Zhang

doi:10.1016/j.saa.2021.119990

A simple dual-response fluorescent probe for imaging of viscosity and ONOO^- through different fluorescence signals in living cells and zebrafish

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 5:260:119990. doi: 10.1016/j.saa.2021.119990. Epub 2021 May 23.

Authors

Chong Wang¹, Wei Shu¹, Qianqian Chen¹, Chunlei Yang¹, Sa Su¹, Mengxu Gao¹, Rubo Zhang², Jing Jing³, Xiaoling Zhang⁴

Affiliations

¹ Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electrophotonic Conversion Materials, Analytical and Testing Center, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China.
² Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electrophotonic Conversion Materials, Analytical and Testing Center, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: zhangrubo@bit.edu.cn.
³ Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electrophotonic Conversion Materials, Analytical and Testing Center, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: hellojane@bit.edu.cn.
⁴ Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electrophotonic Conversion Materials, Analytical and Testing Center, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address: zhangxl@bit.edu.cn.

PMID: 34082351
DOI: 10.1016/j.saa.2021.119990

Abstract

Cellular viscosity is a prominent micro-environmental parameter and peroxynitrite is an essential reactive oxygen special, both of which are involved in various pathological and physiological processes. When the intracellular viscosity is abnormal or the ONOO^- concentration is irregular, the normal function of cells will be disturbed. Herein, we rationally designed and synthesized a novel multichannel fluorescent probe (probe 1) for multichannel imaging of viscosity and peroxynitrite. Probe 1 displayed about 108-fold enhancement as the viscosity increased from 1.005 cP to 1090 cP. Moreover, the fluorescence intensity at 540 nm was quickly increased after adding ONOO^-. It should be noted that probe 1 has high sensitivity, selectivity and low cytotoxicity, which can be successfully employed for the visualization of exogenous and endogenous ONOO^- and imaging viscosity changes in HeLa cells by different fluorescent signals. Furthermore, probe 1 could monitor the change of ONOO^- induced by LPS (lipopolysaccharide) and IFN-γ (interferon-γ) in zebrafish. This result reveals that probe 1 may inspire more diagnostic and therapeutic programs for viscosity-peroxynitrite related diseases shortly.

Keywords: Bioimaging; Fluorescent probe; Peroxynitrite; Viscosity.

MeSH terms

Animals
Fluorescent Dyes*
HeLa Cells
Humans
Peroxynitrous Acid
Viscosity
Zebrafish*

Substances

Fluorescent Dyes
Peroxynitrous Acid