Zirconium ion-mediated assembly of a single quantum dot-based nanosensor for kinase assay

Yueying Li; Qian Liu; Lin Cui; Wenjing Liu; Jian-Ge Qiu; Chun-Yang Zhang

doi:10.1039/d1cc02035h

Zirconium ion-mediated assembly of a single quantum dot-based nanosensor for kinase assay

Chem Commun (Camb). 2021 Jun 29;57(52):6376-6379. doi: 10.1039/d1cc02035h.

Authors

Yueying Li¹, Qian Liu¹, Lin Cui¹, Wenjing Liu², Jian-Ge Qiu², Chun-Yang Zhang¹

Affiliations

¹ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn.
² Department of Internal Medicine, Affiliated Cancer Hospital of Zhengzhou University, Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450000, China. jiangeqiu@zzu.edu.cn.

PMID: 34081069
DOI: 10.1039/d1cc02035h

Abstract

We demonstrate the zirconium ion-mediated assembly of a single quantum dot (QD)-based nanosensor for accurate detection of protein kinases (PKA) and polynucleotide kinases (PNK). This nanosensor is very sensitive with a detection limit of 8.82 × 10-4 U mL-1 for PKA and 1.40 × 10-5 U mL-1 for PNK. Moreover, it can be used to analyze the enzyme kinetic parameters and screen the inhibitors of PKA and PNK, with potential applications in drug discovery and clinical diagnosis.

MeSH terms

Biosensing Techniques / methods*
Carbocyanines / chemistry
Fluorescence Resonance Energy Transfer
HeLa Cells
Humans
Limit of Detection
Polynucleotide 5'-Hydroxyl-Kinase / analysis*
Polynucleotide 5'-Hydroxyl-Kinase / antagonists & inhibitors
Polynucleotide 5'-Hydroxyl-Kinase / metabolism
Protein Kinases / analysis*
Protein Kinases / chemistry
Protein Kinases / metabolism
Quantum Dots / chemistry*
Zirconium / chemistry*

Substances

Carbocyanines
cyanine dye 5
Zirconium
Protein Kinases
Polynucleotide 5'-Hydroxyl-Kinase