S teinernema sandneri n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Poland

Magdalena Lis; Ewa Sajnaga; Marcin Skowronek; Adrian Wiater; Kamila Rachwał; Waldemar Kazimierczak

doi:10.21307/jofnem-2021-051

S teinernema sandneri n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Poland

J Nematol. 2021 May 21:53:e2021-51. doi: 10.21307/jofnem-2021-051. eCollection 2021.

Authors

Magdalena Lis¹, Ewa Sajnaga¹, Marcin Skowronek¹, Adrian Wiater², Kamila Rachwał³, Waldemar Kazimierczak¹

Affiliations

¹ Laboratory of Biocontrol, Production and Application of EPN, Centre for Interdisciplinary Research, Faculty of Natural Sciences and Health, John Paul II Catholic University of Lublin, ul. Konstantynów 1J, 20-708 Lublin, Poland.
² Department of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland.
³ Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, ul. Skromna 8, 20-704 Lublin, Poland.

Abstract

A new species of entomopathogenic nematodes, Steinernema sandneri n. sp., was recovered by baiting from Poland. Its morphological traits indicate that the new species is a member of the feltiae-kraussei group. A body length of 843 (708-965) μm, a more anterior position of excretory pore (56 μm), and the lower D% value (40 vs > 46) discriminate this species from most of the other group members. The first-generation males of S. sandneri n. sp. can be distinguished from the other clade members by a 60 μm long spicule, a relatively long gubernaculum (GS% = 79), and the position of the excretory pore (80 μm). Phylogenetic analysis of the ITS rDNA, D2D3 of 28 S rDNA, and cox1 sequences confirmed that S. sandneri n. sp. is a new species of the feltiae-kraussei group, closely related to S. kraussei and S. silvaticum.

Keywords: 18S rRNA; D2D3 Domain; Description; Entomopathogenic Nematodes; ITS; Mitochondrial cox1; Morphology; Morphometrics; Phylogeny; Steinernema sandneri; Taxonomy.