FeS2 nanoparticles decorated carbonized Luffa cylindrica as biofilm substrates for fabricating high performance biosensors

Ling Liu; Liang Huang; Dengbin Yu; Guangxin Zhang; Shaojun Dong

doi:10.1016/j.talanta.2021.122416

FeS₂ nanoparticles decorated carbonized Luffa cylindrica as biofilm substrates for fabricating high performance biosensors

Talanta. 2021 Sep 1:232:122416. doi: 10.1016/j.talanta.2021.122416. Epub 2021 Apr 19.

Authors

Ling Liu¹, Liang Huang², Dengbin Yu¹, Guangxin Zhang³, Shaojun Dong⁴

Affiliations

¹ Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, The High-tech North District, 4888 Sheng Bei Street, Changchun, 130102, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of the Chinese Academy of Sciences, Beijing, 100049, PR China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
³ Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, The High-tech North District, 4888 Sheng Bei Street, Changchun, 130102, PR China; University of the Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address: zhgx@iga.ac.cn.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; University of the Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address: dongsj@ciac.ac.cn.

PMID: 34074404
DOI: 10.1016/j.talanta.2021.122416

Abstract

A high-performance microbial biosensor was fabricated with a reasonably designed biofilm substrate, where the aerogel of carbonized Luffa cylindrica (LC) was used as the scaffold for loading biofilm and FeS₂ nanoparticles (FeS₂NPs) were employed to modify this aerogel (FeS₂NPs/Gel_LC). The fabricated FeS₂NPs/Gel_LC exhibited a spring-like structure similar with that of the raw LC, which facilitated the linkage of the scaffold and promoted its mechanical strength, and further prolonged the service period of the as-prepared biosensor from few days to two months. Meanwhile, the introduced FeS₂NPs improved the microbial electron transfer of the biofilm and causing an increase in the sensor's signals from 155.0 ± 2.6 to 352.0 ± 17.1 nA and a decrease in the detection limit from 0.95 to 0.38 mg O L^-1 (S/N = 3) for the detection of glucose-glutamic acid (GGA). More important, the FeS₂NPs had been demonstrated to have the capability for modulating a persistent shift of the microbial community with organic pollutant biodegradability. Compared with the Gel_LC, the FeS₂NPs/Gel_LC exhibited a promising performance for measuring the synthetic sewage and real water samples in BOD assay and an increasing inhibition-ratio for detecting 3,5-dichlorophenol (DCP) in toxicity assay. Based on the vast resource and renewability of LC, this work pave a new avenue for developing high-performance microbial biosensors that are expected to be the engineering production.

Keywords: Biofilm; Biomass; Biosensor; High-throughput sequencing; LIVE/DEAD assay; Toxicity assay.

MeSH terms

Biofilms
Biosensing Techniques*
Glucose
Luffa*
Nanoparticles*

Substances

Glucose