Electrochemical oxidation of adenosine-5'-triphosphate on a chitosan-graphene composite modified carbon ionic liquid electrode and its determination

Wei Sun; Jun Liu; Xiuzhen Wang; Tongtong Li; Guangjiu Li; Jie Wu; Liqi Zhang

doi:10.1016/j.msec.2012.06.002

Electrochemical oxidation of adenosine-5'-triphosphate on a chitosan-graphene composite modified carbon ionic liquid electrode and its determination

Mater Sci Eng C Mater Biol Appl. 2012 Oct 1;32(7):2129-2134. doi: 10.1016/j.msec.2012.06.002. Epub 2012 Jun 9.

Authors

Wei Sun¹, Jun Liu², Xiuzhen Wang², Tongtong Li², Guangjiu Li², Jie Wu², Liqi Zhang³

Affiliations

¹ College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, PR China; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. Electronic address: swyy26@hotmail.com.
² College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
³ State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, Wuhan 430074, PR China.

PMID: 34062706
DOI: 10.1016/j.msec.2012.06.002

Abstract

In this paper a new electrochemical method was proposed for the determination of adenosine-5'-triphosphate (ATP) based on a chitosan (CTS) and graphene (GR) composite film modified carbon ionic liquid electrode (CTS-GR/CILE). CILE was fabricated by using ionic liquid 1-butyl-3-methylimidazolium dihydrogen phosphate ([BMIM]H₂PO₄) as the binder, which was further modified by GR and CTS composite. The modified electrode exhibited an excellent electrocatalytic activity toward the oxidation of ATP with the increase of the oxidation peak current and the decrease of the oxidation peak potential. The electrochemical parameters of ATP on CTS-GR/CILE were calculated with the electron transfer coefficient (α) as 0.329, the electron transfer number (n) as 2.15, the apparent heterogeneous electron transfer rate constant (ks) as 3.705×10^-5s^-1 and the surface coverage (Γ_T) as 9.33×10^-10molcm^-2. Under the optimal conditions the oxidation peak current was proportional to ATP concentration in the range from 1.0×10^-6 to 1.0×10^-3M with the detection limit of 0.311μM (S/N=3). The proposed electrode showed excellent reproducibility, stability, anti-interference ability and further successfully applied to the ATP injection sample detection.

Keywords: Adenosine-5′-triphosphate; Carbon ionic liquid electrode; Electrochemistry; Graphene.