ASAP1 regulates the uptake of Mycobacterium tuberculosis H37Ra in THP1-derived macrophages by remodeling actin cytoskeleton

Jia Cui; Guangxin Chen; Zhonghua Zhao; Liqing Wang; Da Wen; Li Xing; Changxin Wu

doi:10.1016/j.tube.2021.102090

ASAP1 regulates the uptake of Mycobacterium tuberculosis H37Ra in THP1-derived macrophages by remodeling actin cytoskeleton

Tuberculosis (Edinb). 2021 Jul:129:102090. doi: 10.1016/j.tube.2021.102090. Epub 2021 May 26.

Authors

Jia Cui¹, Guangxin Chen², Zhonghua Zhao², Liqing Wang², Da Wen², Li Xing³, Changxin Wu⁴

Affiliations

¹ Institutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, Shanxi province, China; Department of Microbiology, Changzhi Medical College, 161 Jiefang Road, Changzhi, 046000, Shanxi province, China.
² Institutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, Shanxi province, China.
³ Institutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, Shanxi province, China; Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan, 030006, China; Shanxi Provincial Key Laboratory for Prevention and Treatment of Major Infectious Diseases, Taiyuan, 030006, China. Electronic address: xingli107@gmail.com.
⁴ Institutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, Shanxi province, China; Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan, 030006, China; Shanxi Provincial Key Laboratory for Prevention and Treatment of Major Infectious Diseases, Taiyuan, 030006, China. Electronic address: cxw20@sxu.edu.cn.

PMID: 34058694
DOI: 10.1016/j.tube.2021.102090

Abstract

Tuberculosis is initiated by the entry of Mycobacterium tuberculosis (Mtb) into macrophages in the lungs. A study of the cellular factors responsible for the entry of Mtb into host cells will potentially benefit the development of therapeutic treatments or preventive agents against Mtb infection. Using human THP1-derived macrophages as a model, we found that infection of Mtb H37Ra transiently reduced the level of ASAP1, an ADP ribosylation factor (Arf)-GTPase activating protein. Furthermore, knockdown of ASAP1 increased the efficiency of H37Ra entry into the cell and altered the status of actin remodeling as indicated by the enhanced aggregation of F-actin and the increased numbers of vinculin- and paxillin-rich puncta. Collectively, the results in this report identified ASAP1 as a regulator controlling the entry of Mtb H37Ra into macrophage by remodeling actin cytoskeleton.

Keywords: ASAP1; Actin cytoskeleton; H37Ra; Mycobacterium; THP-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / metabolism*
Actins
Adaptor Proteins, Signal Transducing / metabolism*
Gene Knockdown Techniques
Humans
Macrophages / microbiology*
Mycobacterium tuberculosis*
THP-1 Cells
Tuberculosis

Substances

ASAP1 protein, human
Actins
Adaptor Proteins, Signal Transducing