Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells

Methods Mol Biol. 2021:2308:73-81. doi: 10.1007/978-1-0716-1425-9_6.

Abstract

Flow cytometry has been widely used to detect a single event by means of multiparametric fluorescence measurements. Here we describe a method to analyze subsets of hematopoietic stem and progenitor cells isolated from long bones of mice. We further show that this method allows for comparing JAM-C protein expression between subsets of hematopoietic stem and progenitor cells.

Keywords: Bone marrow; Flow cytometry; Hematopoietic stem cells; Mouse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Cell Adhesion Molecules / metabolism*
  • Cell Lineage
  • Cell Separation*
  • Flow Cytometry*
  • Hematopoietic Stem Cells / metabolism*
  • Immunoglobulins / metabolism*
  • Mice
  • Multipotent Stem Cells / metabolism*
  • Phenotype

Substances

  • Biomarkers
  • Cell Adhesion Molecules
  • Immunoglobulins
  • Jam3 protein, mouse