Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

Xianhong Wang; Yashuang Guo; Yamin Du; Ziling Yang; Xinzhong Huang; Ni Hong; Wenxing Xu; Guoping Wang

doi:10.3897/mycokeys.80.63816

Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China

MycoKeys. 2021 May 18:80:77-90. doi: 10.3897/mycokeys.80.63816. eCollection 2021.

Authors

Xianhong Wang¹, Yashuang Guo¹, Yamin Du¹, Ziling Yang¹, Xinzhong Huang², Ni Hong^{1

3}, Wenxing Xu^{1

3}, Guoping Wang^{1

3}

Affiliations

¹ Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China Huazhong Agricultural University Wuhan China.
² Research Institute of Pomology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, China Key Laboratory of Horticultural Crop Biology and Germplasm Creation of the Ministry of Agriculture Wuhan China.
³ Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, Wuhan, Hubei, 430070, China Research Institute of Pomology, Fujian Academy of Agricultural Sciences Fuzhou China.

Abstract

Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

Keywords: Diaporthe; Constriction canker; Prunus persica; multi-gene phylogeny; taxonomy; two new taxa.

Xianhong Wang, Yashuang Guo, Yamin Du, Ziling Yang, Xinzhong Huang, Ni Hong, Wenxing Xu, Guoping Wang.

Grants and funding

This work was financially supported by the earmarked fund for Pear Modern Agro-Industry Technology Research System (CARS-28-16) of the Chinese Ministry of Agriculture and the Fundamental Research Funds for the Central Universities (no. 2662016PY107).