Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum

Ghassan Mustafa; Charles L Cai; Darren Bodkin; Jacob V Aranda; Kay D Beharry

doi:10.1016/j.prostaglandins.2021.106565

Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum

Prostaglandins Other Lipid Mediat. 2021 Aug:155:106565. doi: 10.1016/j.prostaglandins.2021.106565. Epub 2021 May 26.

Authors

Ghassan Mustafa¹, Charles L Cai¹, Darren Bodkin², Jacob V Aranda³, Kay D Beharry⁴

Affiliations

¹ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY, USA.
² Department of Pediatrics, State University of New York, Downstate Medical Center, Brooklyn, NY, USA.
³ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY, USA; Department of Ophthalmology, State University of New York, Downstate Medical Center, Brooklyn, NY, USA.
⁴ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY, USA; Department of Ophthalmology, State University of New York, Downstate Medical Center, Brooklyn, NY, USA. Electronic address: kay.beharry@downstate.edu.

PMID: 34051366
DOI: 10.1016/j.prostaglandins.2021.106565

Abstract

Intermittent hypoxia (IH) is associated with the pathogenesis of necrotizing enterocolitis (NEC). We tested the hypothesis that early supplementation with antioxidants and/or fish oil protects the terminal ileum from oxidative injury induced by neonatal IH. Newborn rats were exposed to neonatal IH from birth (P0) until P14 during which they received daily fish oil, coenzyme Q10 (CoQ10), glutathione nanoparticles (nGSH), fish oil + CoQ10, or olive oil. Pups were then placed in room air from P14 to P21 with no further supplementation. Terminal ileum was assessed for IH-induced injury and inflammatory biomarkers. Neonatal IH induced severe damage consistent with NEC, and was associated with oxidative stress and elevations in PGE₂, PGF_2α, TxB₂, NOS-2 and TLR-4, effects that were ameliorated with nGSH and combination CoQ10+fish oil. Early postnatal supplementation with antioxidants and/or fish oil during neonatal IH may be favorable for preserving gut integrity and reducing oxidative injury.

Keywords: Coenzyme Q10; Fish oil; Glutathione nanoparticles; Neonatal intermittent hypoxia; Prostanoids; Terminal ileum.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Fish Oils*

Substances

Fish Oils

Grants and funding

U54 HD071594/HD/NICHD NIH HHS/United States