Assessment of the impact of hydrolysis on bound sulfonamide residue determination in honey using stable isotope dilution ultrahigh performance liquid chromatography tandem mass spectrometry

Yan Zhang; Xiu Qin Li; Zhen Guo; Xia Zhou; Shuang Qing Li; Xiao Min Li; Hui Jiao; Qing He Zhang

doi:10.1016/j.foodchem.2021.130094

Assessment of the impact of hydrolysis on bound sulfonamide residue determination in honey using stable isotope dilution ultrahigh performance liquid chromatography tandem mass spectrometry

Food Chem. 2021 Nov 1:361:130094. doi: 10.1016/j.foodchem.2021.130094. Epub 2021 May 13.

Authors

Yan Zhang¹, Xiu Qin Li², Zhen Guo¹, Xia Zhou¹, Shuang Qing Li¹, Xiao Min Li¹, Hui Jiao¹, Qing He Zhang³

Affiliations

¹ Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China.
² Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China. Electronic address: lixq@nim.ac.cn.
³ Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China. Electronic address: zhangqh@nim.ac.cn.

PMID: 34029905
DOI: 10.1016/j.foodchem.2021.130094

Abstract

In this study, an analytical method based on isotope dilution-liquid chromatography tandem mass spectrometry (ID-LC-MS/MS) was developed as a candidate reference method for the determination of sulfonamides (SAs) in honey. To guarantee the accuracy and authenticity, the impact of hydrolysis on bound SA residues was first investigated by enabling (i) identification of sugar-bound SAs, (ii) clarifying the binding reaction rule between the SAs and sugar, (iii) detection of free SAs and sugar-bound SAs, and (iv) preparation of SA-contaminated honey. Thus, the efficiency of different hydrolysis conditions was assessed by comparing the bound SA content before and after hydrolysis. In addition, optimization of the sample pretreatment procedures and LC conditions to minimize matrix effects by separation from significant matrix interferences was also performed. Satisfactory results in terms of hydrolysis efficiency (approximately 88.3%-99.2%), extraction efficiency (84.2%-105.3%), recovery (95.9%-103.1%), and limit of quantification (0.6-1.5 μg·kg^-1) were obtained.

Keywords: Assessment; Bound sulfonamide residues; Honey; Hydrolysis efficiency; ID-LC-MS/MS.

MeSH terms

Chromatography, High Pressure Liquid / methods*
Food Analysis / methods*
Honey / analysis*
Hydrolysis
Indicator Dilution Techniques
Isotopes
Reproducibility of Results
Sulfonamides / analysis*
Sulfonamides / chemistry
Tandem Mass Spectrometry / methods*

Substances

Isotopes
Sulfonamides