Spatial multi-omics sequencing for fixed tissue via DBiT-seq

STAR Protoc. 2021 May 11;2(2):100532. doi: 10.1016/j.xpro.2021.100532. eCollection 2021 Jun 18.

Abstract

This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics and spatial analysis of a targeted panel of proteins. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

Keywords: Genomics; RNA-seq; Sequencing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / analysis
  • DNA / chemistry
  • DNA / genetics
  • DNA Barcoding, Taxonomic / methods
  • Equipment Design
  • Gene Expression Profiling / methods*
  • Genomics / instrumentation
  • Genomics / methods*
  • Microfluidic Analytical Techniques / instrumentation
  • Microfluidic Analytical Techniques / methods
  • Sequence Analysis, DNA / methods*
  • Transcriptome / genetics

Substances

  • DNA