Inhibitory effects of Lactobacillus brevis KU15153 against Streptococcus mutans KCTC 5316 causing dental caries

Hye Ji Jang; Jong Ha Kim; Na-Kyoung Lee; Hyun-Dong Paik

doi:10.1016/j.micpath.2021.104938

Inhibitory effects of Lactobacillus brevis KU15153 against Streptococcus mutans KCTC 5316 causing dental caries

Microb Pathog. 2021 Aug:157:104938. doi: 10.1016/j.micpath.2021.104938. Epub 2021 May 20.

Authors

Hye Ji Jang¹, Jong Ha Kim¹, Na-Kyoung Lee¹, Hyun-Dong Paik²

Affiliations

¹ Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, South Korea.
² Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, South Korea. Electronic address: hdpaik@konkuk.ac.kr.

PMID: 34022360
DOI: 10.1016/j.micpath.2021.104938

Abstract

This study determined the inhibitory effect of Lactobacillus brevis KU15153 against cariogenic Streptococcus mutans KCTC 5316. Antimicrobial activity, auto-aggregation, cell surface hydrophobicity, exopolysaccharides (EPS) production, biofilm formation, and morphological changes were assessed in the presence of L. brevis KU15153. L. brevis KU15153 exhibited the highest antimicrobial activity against S. mutans KCTC 5316 (28.67 ± 4.16 mm). Auto-aggregation (38.32%), cell surface hydrophobicity (27.08%), and EPS production rate (58.52%) of S. mutans KCTC 5316 slightly decreased upon treatment with L. brevis KU15153. Additionally, crystal violet stanning and scanning electron microscopy confirmed the L. brevis KU15153-mediated inhibition of biofilm formation by S. mutans KCTC 5316 in comparison to that observed in the negative control (untreated S. mutans KCTC 5316). These results indicate that the L. brevis KU15153 could be used as a potential probiotic for maintaining oral health.

Keywords: Antibiofilm effect; Anticavity effect; Antimicrobial effect; Oral health; Probiotics.

MeSH terms

Biofilms
Dental Caries*
Humans
Levilactobacillus brevis*
Probiotics*
Streptococcus mutans