Activation of palindromes on a degradable modular grafting probe enables ultrasensitive detection of microRNAs

Chem Commun (Camb). 2021 Jun 15;57(48):5941-5944. doi: 10.1039/d1cc01150b.

Abstract

This work describes a single-stranded degradable modular grafting probe for analyzing microRNA-21. In the system, the exonuclease activity of phi29 polymerase restrains the SYBR Green I/ssDNA induced background. The palindrome activation caused remarkable target fluorescence. The detection limit was achieved as 0.26 fM, showing potential in biochemical analysis.

MeSH terms

  • Benzothiazoles / chemistry*
  • Benzothiazoles / metabolism
  • DNA, Single-Stranded / chemistry*
  • DNA, Single-Stranded / metabolism
  • Diamines / chemistry*
  • Diamines / metabolism
  • Exodeoxyribonucleases / chemistry*
  • Exodeoxyribonucleases / metabolism
  • Fluorescence
  • Humans
  • MicroRNAs / analysis*
  • MicroRNAs / metabolism
  • Nucleic Acid Amplification Techniques
  • Quinolines / chemistry*
  • Quinolines / metabolism

Substances

  • Benzothiazoles
  • DNA, Single-Stranded
  • Diamines
  • MIRN21 microRNA, human
  • MicroRNAs
  • Quinolines
  • SYBR Green I
  • Exodeoxyribonucleases