Abstract
This work describes a single-stranded degradable modular grafting probe for analyzing microRNA-21. In the system, the exonuclease activity of phi29 polymerase restrains the SYBR Green I/ssDNA induced background. The palindrome activation caused remarkable target fluorescence. The detection limit was achieved as 0.26 fM, showing potential in biochemical analysis.
MeSH terms
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Benzothiazoles / chemistry*
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Benzothiazoles / metabolism
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DNA, Single-Stranded / chemistry*
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DNA, Single-Stranded / metabolism
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Diamines / chemistry*
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Diamines / metabolism
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Exodeoxyribonucleases / chemistry*
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Exodeoxyribonucleases / metabolism
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Fluorescence
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Humans
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MicroRNAs / analysis*
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MicroRNAs / metabolism
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Nucleic Acid Amplification Techniques
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Quinolines / chemistry*
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Quinolines / metabolism
Substances
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Benzothiazoles
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DNA, Single-Stranded
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Diamines
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MIRN21 microRNA, human
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MicroRNAs
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Quinolines
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SYBR Green I
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Exodeoxyribonucleases