Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro

STAR Protoc. 2021 May 3;2(2):100517. doi: 10.1016/j.xpro.2021.100517. eCollection 2021 Jun 18.

Abstract

Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions. For complete details on the use and execution of this protocol, please refer to Wabitsch et al. (2021).

Keywords: Cancer; Flow Cytometry/Mass Cytometry; Immunology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects*
  • Cytokines / immunology*
  • Drug Evaluation
  • Hydroxychloroquine / pharmacology*
  • Immunologic Factors / pharmacology*
  • Lymphocytes / immunology*
  • Mice
  • Mice, Transgenic

Substances

  • Cytokines
  • Immunologic Factors
  • Hydroxychloroquine