Abstract
We report the design and evaluation of pH responsive luminescent europium(iii) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.
MeSH terms
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Coordination Complexes / chemical synthesis
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Coordination Complexes / chemistry*
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Europium / chemistry*
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Exenatide / pharmacology
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Glucagon-Like Peptide-1 Receptor / agonists
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Glucagon-Like Peptide-1 Receptor / analysis*
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HEK293 Cells
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Humans
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Hydrogen-Ion Concentration
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Luminescent Agents / chemical synthesis
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Luminescent Agents / chemistry*
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Luminescent Measurements
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Optical Imaging
Substances
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Coordination Complexes
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GLP1R protein, human
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Glucagon-Like Peptide-1 Receptor
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Luminescent Agents
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Europium
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Exenatide