Accurate mass screening of pesticide residues in wine by modified QuEChERS and LC-hybrid LTQ/Orbitrap-MS

Christina I Kosma; Ourania L Koloka; Triantafyllos A Albanis; Ioannis K Konstantinou

doi:10.1016/j.foodchem.2021.130008

Accurate mass screening of pesticide residues in wine by modified QuEChERS and LC-hybrid LTQ/Orbitrap-MS

Food Chem. 2021 Oct 30:360:130008. doi: 10.1016/j.foodchem.2021.130008. Epub 2021 May 5.

Authors

Christina I Kosma¹, Ourania L Koloka¹, Triantafyllos A Albanis², Ioannis K Konstantinou³

Affiliations

¹ Department of Chemistry, University of Ioannina, Ioannina 45110, Greece.
² Department of Chemistry, University of Ioannina, Ioannina 45110, Greece; Institute of Environment and Sustainable Development, University Research Center of Ioannina (URCI), Ioannina 45110, Greece.
³ Department of Chemistry, University of Ioannina, Ioannina 45110, Greece; Institute of Environment and Sustainable Development, University Research Center of Ioannina (URCI), Ioannina 45110, Greece. Electronic address: iokonst@uoi.gr.

PMID: 34000630
DOI: 10.1016/j.foodchem.2021.130008

Abstract

In this research, a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure and Ultra-High Performance Liquid Chromatography-Orbitrap-Mass Spectrometry (UHPLC-Orbitrap-MS), were combined to obtain a sensitive and rapid method for the determination of multiclass pesticides in white and red wines. The optimization strategy involved the selection of buffering conditions, by applying different QuEChERS procedures and sorbents for the cleanup step in order to achieve acceptably high recoveries and low co-extractives in the final extracts. Identification was based on both accurate mass and retention time, while further confirmation was achieved by MS fragmentation. The method was evaluated in terms of linearity, recovery, precision, limit of detection (LOD) and quantification (LOQ), matrix effects (ME) and expanded uncertainty. The validated method was successfully applied to real samples (home-made and commercial) revealing the presence of two selected fungicides, in relatively low levels compared to the MRLs defined by the EU for vinification grapes.

Keywords: Analytical methodology; Liquid chromatography–LTQ Orbitrap mass spectrometry; Pesticides; QuEChERS; Uncertainty; Wine.

MeSH terms

Chromatography, Liquid / methods*
Limit of Detection
Mass Screening
Mass Spectrometry / methods*
Pesticide Residues / analysis*
Wine / analysis*

Substances

Pesticide Residues