In situ analysis of biomolecular interactions on nano/microparticles in formulations

J Pharm Biomed Anal. 2021 Jul 15:201:114130. doi: 10.1016/j.jpba.2021.114130. Epub 2021 May 7.

Abstract

Nano/microparticle-based formulations are attracting more and more attention in the drug and vaccine formulation field. Analytical methodologies are needed for analyzing the function of the protein molecules before and after their adsorption onto particles. Here, we report a fluorescence imaging-based high content analysis (HCA) for analyzing bimolecular interactions on the particle surface. Subtle conformational alteration of antigen epitope overlapping were shown to be feasible using this microplate-based HCA method. This HCA is highly amenable for automation, making it a method of choice for early formulation development as well as for stability test of products in late stage development.

Keywords: Antigenicity; Epitope mapping; Hepatitis B surface antigen; High content analysis; Monoclonal antibody.

MeSH terms

  • Adsorption
  • Antigens*
  • Epitopes
  • Vaccines*

Substances

  • Antigens
  • Epitopes
  • Vaccines